Time effects on accuracy of homemade urease test in diagnosis of H. Pylori

Research Article                                                                         

Muthanna Medical Journal
Volume 2, Issue 2, December 2015, Pages 70–75
 

Bashar Ali Saeed1*

*Corresponding Author: Bashar Ali Saeed: bashar007ali@yahoo.com
1Department of Medicine, Faculty of Medicine, MuthannaUniversity Received 01 August 2015; Accepted 29 November 2015; Published 29 December 2015 Copyright © 2015 BS.
This is article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

To evaluate the effect of test duration on the sensitivity and specificity of positive urease test for the detection of Helicobacter pylori in patient with duodenal ulcer and gastropathy, and to evaluate the difference of the results of rapid urease test in patients with duodenal ulcer compared to patients with gastropathy. 117 patient "75 patient  with duodenal ulcer group (A) and 42 patient with gastropathy group (B)" were included in the study, two sets of gastric biopsies "each contain one antral and one corpus biopsy" were obtained from each patient, one set used to obtain urease test which was read at 30 minutes, 2, 10 and 24 hour after biopsy insertion into the reagent, and the other set used for histopathological examination. 68/75 (90%) patients were tested positive for Helicobacter Pylori by histopathological examination in group (A), in comparison to 35/42 (83%) patient in group (B). The sensitivities of the urease test at 30 min, 2, 10 and 24 hours in group (A) were 45%, 62%, 73% and 99% respectively, corresponding figures for the specificity were 92%, 83%, 80% and 80% respectively. While in group (B) the sensitivities were 42%, 59%, 69%, and 95% respectively, and the specificity was 96%, 88%, 83%, and 81% respectively. The optimum duration of incubation of urease test for a good sensitivity was 24 hours; rapid urease test does not correlate to disease severity.
Keywords: Helicobacter Pylori, Urease test, Duodenal ulcer, Gastropathy

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