Mohammed Abdul Reda Yassen1*
Methicillin-resistant Staphylococcus aureus is the major cause of healthcare-associated bacteremia in most of Hospital and it increased risk of infection, morbidity and mortality especially, when associated vancomycin resistance in same infection. In this study 42 S. aureus were isolated from wound infection patients in Diwaniya hospital and S. aureus was isolated by selective medium out form 50 swab samples. The PCR assay was used for direct detection of methicillin (mecA) and vancomycin (van) antibiotics resistance gene in 42 S. aureus isolates. The results show only 5 isolates (11%) were have single methicillin resistance and there no single vancomycin resistance in all isolates. While the PCR results show there found 3 isolates (7.1%) were have association resistance methicillin and vancomycin resistance. But all methicillin resistance was 12 isolates (19%). In conclusion, we conclude that PCR assay can be used as highly sensitive and specific in detection of methicillin and vancomycin resistance gene, and Vancomycin-resistant can be associated with Methicillin resistance in Staphylococcus aureus isolated from wound infection.
Keywords: Methicillin; Vancomycin; Wound; Staphylococcus aureus