Evaluation the benefit of kalazar detect rapid test for detection of visceral leishmaniasis in Al-Diwaniah province/Iraq

Muthanna Medical Journal
Volume 4, Issue 1, June 2017, Pages 50-60
http://dx.doi.org/10.18081/2410-4590/2017-50-60

Research Article
Abdulaziz Wannas Abd, Ahmed Kadem Challab

email; abdulazizwannas@yahoo.com*¹Al Muthanna University/ College of Science
Received 13 March 2017, Accepted 20 May 2017, Available online 30 June 2017
This is article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2017 AW

Abstract

The aim of study is to determine the benefit of In Bios kala-azar detect rapid test in comparative with IFA test in cases with clinical suspicion of visceral leishmaniasis. Eighty  four patients with clinical diagnosis of  were studied in children and maternity teaching hospital in Al-Diwaniah/Iraq  for children  and all specimens were checked by using the Kala-azar  detect  rapid test and rechecked after that by using immunofluorescence antibody test, after  that  the  patients  sera  were  sent  for immune-flourescent antibody tests test to confirm or to exclude the diagnosis of the kala-azar. In a current study of eight four (84) patients were clinically suspected as kala-azar (visceral leishmaniasis) using the main two common laboratory investigation (InBios kala-azar detect rapid test and IFA test). The diagnosis of visceral leishmaniasis depend on clinical suspicion of the common signs and symptoms of the disease in areas highly endemic with kala-azar; hepatomegaly in 40/84 patients (7.6%) and splenomegaly founded in 81/84 (96.4%). Fever and pallor were detected in almost all patients through the first clinical attack of visceral leishmaniasis 84/84 (100%). Kala-azar detect rapid test truly  positive only in about 72/84  with  sensitivity  reach  (85.71%) the  in  compares  to  other test;  direct immuno-flourecenc antibody (IFA) test  in which the sensitivity of the test reach to (98.8%); and the InBios kala-azar detect rapid test appear positive in  only one case from control  group thirty (30), diagnosed clinically as a typhoid fever  in which the specificity of the test were (96.9 %). In conclusion, the kala-azar detect rapid test is a sensitive, not invasive, rapid, easy to give result within minutes and low cost diagnostic method to be used for the diagnosis of kala-azar but should not use as  the  sole  criterion  for the  diagnosis   of the visceral leishmaniasis  unless the  signs  and  symptoms  present.

Keywords: Kala-azar; Immunoflourecenc antibody (IFA); Leishmaniasis

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