Antimicrobial activity of vaginal Lactobacillus against urogenital pathogens

Muthanna Medical Journal
Volume 4, Issue 2, December 2017, Pages 157-167

Research Article

Suhad Faisal Hatem Al-Mugdadi¹,

Ishraq Hasan Elewi,

Zainab Farooq Shafeeq Al-Ryyis,

Hala Muaed, Mays Ali

* Correspondence author:
¹University of Al-Mustansiriyah/ College of Pharmacy/Department of Clinical Laboratories sciences
Received 12 August 2017, Accepted 30 November 2017, Available online 27 December 2017
This is article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2017 SA


Lactobacillus is one of the most important genera of Lactic Acid Bacteria (LAB). Lactobacilli present in a healthy vagina are part of normal bacterial microbiota and protect the host from urogenital infections by maintaining a low pH. Important features of probiotic lactobacilli are to achieve antagonistic activity against bacterial pathogens because of their capacity to produce lactic acid and other organic acids, H2O2 and bacteriocin. This study aimed to studying the effect of  Lactobacillus spp. isolated from healthy vagina as aprobiotic against many clinical bacterial isolates  from urogenital tract infection. Susceptibility to Five antibiotics, such as amikacin (AK), Tobramycin (TOB), Pipracillin (PRL), Nalidixic acid (NA) and Gentamycin(GN) was achived on all pathogenic bacteria isolated from urine and vagina. Two fold dilutions of Lactobacillus sp. were prepared (1 × 108, 1 × 104  and 1 × 102) CFU/ml to determine antibacterial activity against urogenital   bacteria isolates using agar well diffusion method. N. gonorrhoeae from vagaina was affected by all these dilutions of Lactobacillus sp.  with inhibition zones 10, 8 and  8 mm respectively. While inhibition zones of S. aureus and Candida albiccans from vagaina were 12mm in dilution (1 × 104  ) and 6 mm in dilution(108 ) respectively. In addition there was no biological activity  of Lactobacillus sp.against bacteria isolated from urine. The effectiveness of natural lactobacilli from vagina consider  as a good therapeutics against bacterial illness of humans . We need future study to purification the active component like  bacteriocin or other organic acids from this bacteria.

Key words: Antibiotics; lactobacilli,vagina; Probiotic; Agar well diffusion method

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