The Effects of different type of cryopreservation on DNA in testicular tissue of mice

Muthanna Medical Journal
Volume 5, Issue 2, December 2018, Pages 115-134
http://dx.doi.org/10.18081/2410-4590/2018-115-134

Research Article

Hakeem JK¹, Estabraq A Al-Wasiti2*, Alaa NH, Anam R. Al-Salihi³

* Correspondence author: estabraqalwasiti@yahoo.com
¹Institute of Embryology Research & Infertility treatment, Al-Nahrain University, Baghdad, Iraq 
2College of Medicine, Al-Nahrain University, Baghdad, Iraq
³Ministry of Health and environment of Iraq, Alkarkh, Baghdad, Iraq
Received 21 July 2018, Accepted 31 October 2018, Available online 14 December 2018
This is article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2018 EW

Research Article

Cryopreservation is a process where cells or whole tissues are preserved by cooling to low sub-zero temperatures, such as (typically) -80°C or -196°C and this process use extensively in programs of in vitro fertilization (IVF). This study is an attempt to evaluate DNA fragmentation in the cell after cryopreservation/thawing cycle when using different types of cryoprotectant (CPA), sixty mature fertile male mice were used in the current study, the mean age of these mice were ten weeks. Fifteen of them were considered as control group and the rest (forty five) as cryopreserved group, this group was divided into three subgroup according to the type of cryoprotectant (glycerol, 1, 2 propanediol and dimethylsulfoxide), each subgroup composed from fifteen mice. The testes were cryopreserved for six weeks then DNA fragmentation assay was done by single cell gel electrophoresis (comet assay). Comet results of the present study showed a highly significant (P<0.0001) increase in DNA damage in the cryopreserved testis (33.26%, 38.8% and 30.6% represent cryoprotectant glycerol, 1, 2 propanediol and dimethylsulfoxide respectively) compared with control group (23.06%) after six weeks of cryopreservation. From the results of the present study, it was concluded that there was increase the levels of DNA fragmentation in the testicular tissue after cryopreservation /thawing cycle differs according to the type of cryoprotectant, Dimethylsulfoxide cryoprotectant provides good protection to the testicular tissue and DNA in cryopreservation /thawing cycle than glycerol and 1, 2 propanediol.

Keywords: Cryopreservation; DNA; Testicular tissue of mice

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